The Initials Project: JR-CSF and JR-FL

29 October 2013 by Nathalia Holt, posted in The Initials Project

For two years I worked with a strain of HIV called JR-CSF. Although I was well versed in how the virus behaved in human cells grown in a flask, I had no idea where the strain came from or how it received its name. The initials project was inspired by the work of Rebecca Skloot and her investigation of Henrietta Lacks, known in the lab only as HeLa. In this series we’ll explore the people and science that made the cells, viruses and reagents behind the initials and acronyms used everyday in labs worldwide. First up is the story of J.R.

 

Yoshio Koyanagi wrote the initials on the side of the vial in tiny print: JR-CSF. It was the first year of his postdoctoral training and, in his own words; he “didn’t know the rules of nomenclature.” Instead he named the virus based on where it came from.  The name was simple and descriptive. He took the samples from a man whose initials were J.R. Then he added which part of the body they were isolated from. JR-CSF came from the filtered liquid of J.R.’s cerebrospinal fluid. JR-FL, on the other hand, was isolated from the frontal lobe of J.R.’s brain.

This was the first HIV positive patient Yoshio had ever isolated virus from. The year was 1986. On a warm evening in June, J.R. passed away. His disease was rapid. He had AIDS encephalopathy, a horrible disorder where the virus shuts down the brain. In his last days the virus left him with a crippling dementia. As if that wasn't enough, J.R. also suffered from a cancer commonly found in those with HIV called Kaposi’s sarcoma. It left red lesions across his body and made it difficult for him to breathe. He wasn’t alone in his illness. His partner had recently succumbed to the same unexplainably rapid AIDS disease.

 

It was the early days of the epidemic. Two years earlier, in 1984, HIV had been identified as the cause of AIDS. Despite this knowledge, there was no treatment available. Doctors could offer little but supportive care. In 1987 the FDA approved AZT, the first drug effective against HIV. It was a year too late for J.R.

 

Yoshio went to graduate school at Kyoto University in Japan before coming to Irvin Chen’s lab at UCLA for his postdoc. He entered the autopsy room on June 11, 1986. Yoshio was interested in HIV and the central nervous system. When he took the virus from J.R.’s brain and spinal fluid he couldn’t know that the samples he was extracting would become critically important to HIV research.

 

What makes J.R.’s samples so special is that even though they were both extracted from the central nervous system, one from the brain and one from the cerebrospinal fluid, they are genetically distinct. Although they came from the same person, they are two viruses. They even infect different cells. The virus originating from the brain can infect macrophages, an immune cell that protects us by eating up things like bacteria and dead cells. The description of J.R.’s viruses, published in 1987, was an early glimpse inside HIV’s diverse and pathogenic world. But the real gift J.R. gave us is the unique ability of his viruses to behave as aggressively in a lab dish as they once did in his body. The pathogenic nature of his viruses is partly determined by the HIV strain itself but also selected from the pressure a person’s immune system places on the virus. Make no mistake; J.R.’s viruses are valuable because he made them that way.

 

In the 25 years since J.R.’s viruses were isolated, they have been grown in hundreds of laboratories all over the world. The virus has become a respected lab-adapted strain that can be counted on to kill human cells and cell lines in culture, producing large quantities of itself in the process. The strain has been used in countless studies: from vaccine development to microbicides to eradication research. There’s hardly an area of HIV research that J.R. hasn’t had some role in.

 

Today thousands of HIV researchers around the world work with JR-CSF and JR-FL on a daily basis without any thought of the person behind the initials. HIV and J.R. are both literally and figuratively intertwined. Even after billions of rounds of replication, the genomes of JR-CSF and JR-FL hold the imprint of the person who carried them. Part of J.R. is forever wrapped up in the replicating virus. Similarly, the initials J.R. have become part of HIV’s identity, indistinguishable from the virus for those familiar with the samples. Every day students and postdocs thaw tiny vials with the labels JR-CSF and JR-FL neatly printed on opaque labels. They grow the virus in a warm incubator, writing J.R. on the side of flasks, tubes, plates. They print J.R. in their lab notebooks and then type his initials again in their research papers. His initials are spoken aloud in meetings, presentations, and the hushed tones that herald new data. He is everywhere and yet nowhere. Thank you J.R.

 

Interested in working with J.R.’s samples? You can order them from the NIH repository here

Yoshio Koyanagi, MD/PhD is a professor at Kyoto University and still works with JR-CSF and JR-FL you can find his lab here


34 Responses to “The Initials Project: JR-CSF and JR-FL”

  1. MADAM VICKY Reply | Permalink

    I have been suffering hardship from HIV/AIDS since 7yrs now, and i happen to have 2 kids for my husband, and now we cannot proceed to have another kids all because of my disease and now i have do all what a human like i and my husband can do just to get my disease healed, i have went to several places to seek for help not even one person could ever help, until i melt a comment on the daily news paper that was commented by Desmond about how this powerful traditional doctor help him get cured of the disease (HIV-AIDS) " my fellow beloved" i firstly taught having a help from a spiritual traditional healer was a wrong idea, but i think of these, will i continue to stress on these disease all day when i have someone to help me save my life?" so i gather all my faiths and put in all interest to contact him through his Email address at hivcurecenter@gmail.com , so after i have mailed him of helping get my disease cured, he respond to me fast as possible that i should not be afraid, that he is a truthful and powerful doctor which i firstly claimed him to be. So after all set has been done, he promise me that i will be healed but on a condition that i provide him some items and obeyed all his oracle said. I did all by accepting his oracles fact and only to see that after some weeks of taking his herbal medicine i notice some changes in my body system and i went for check up the day he ask me to go for check up to confirm if the sickness was still there,to my greatest surprise i could not find any sickness in my body i was first shocked and later arise to be the happiest woman on earth after i have concluded my final test on the hospital by my doctor that i am now HIV- Negative. My papers for check are with me and now i am happy and glad for his miraculous help and power.
    With these i must tell everyone who might seek for any help, either for HIV cure or much more to contact him now at these following email now,
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    " sir thank you so much for your immediate cure of my disease, i must say a big thanks for curing my disease, i owe you in return. Thanks and be blessed sir.
    MADAM VICKY

    • shally mills Reply | Permalink

      Good day everyone,I AM FROM SOUTH AFRICA I have been suffering from HIV/Aids for over 4 years now, and suddenly, i have spent all my money all to make sure i get healthy all day, I came in contact with a traditional doctor also known as Dr.Trust on a newspaper. who has help much people to cure their HIV and AIDS disease, firstly i taught it was a joke until i contacted him.and he said that if i am ready for this work, i told him yes, and he collected my details and told me that the spell will only take two days.i was unhealthy ling surprise. And truly he called me to quickly go for a medical checkup, which I did, only to find out that I was not with any HIV disease anymore, my friends and families who left me before run back to me. I now found out that God in Heaven is using this man to bless and heal us all, he is a great and powerful man, again I say to you sir, that God Almighty will uplift you and your great work you did for I and other people. Please i will sincerely advice all HIV patient to contact this great powerful man called Dr Trust for your solutions. Ultimatespellcast@gmail.com or Ultimatespellcast@yahoo.com or call him +2348156885231 He is the best spell caster to work with,i promise you that you will get your problems solved. ...

    • kevin anthony Reply | Permalink

      Reasons Why I Can Say Love Spells And other Kinds Of Spell Works
      Reading about spell is not new to anyone who uses the internet frequently.More accurately, most of us depend greatly on the internet for information's and ways to fix things.It can be relationship related, or finance related or any kind of problem you can think of.So in all words we try everything we read to fix it which might just include spell casting.I know this sounds crazy but believe me spell casting is just as real as any other thing you can think of. It just turned out that the internet is flooded with a lot of fake making it is very difficult to separate the "shaft from the wheat" fake from real so here are reason that i think will convince positively about spell casting.
      Even before any of us reading this article was brought into exist those before us and before them and before those before them relied on spell for healing, protection, blessing and all other thing and guess what? they never complained about the failure maybe because there where no fakes then or those who asked the spell casters help completed the process rather than not seeing it through to the end.
      A lot of supernatural thing happens around us.We are either too blind to see it or we just let science explain it for us.We think anything that science can't explain is left to be thought as nothing.
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  2. dr ekpen Reply | Permalink

    dr ekpen

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  3. Morgan harriet Reply | Permalink

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  4. Jessica Frank Reply | Permalink

    This is a testimony that i will tell to everyone to hear. I am Jessica Frank? have been married for three years and on the fifth years of my marriage, another woman had a spell to take my lover away from me and my husband left me and the kids and we have suffered for 2 years until i met Dr. Ekpiku of the Ekpikuspelltemple@live.com where so many people have been helped and i decided to give him a try to help me bring my lover back home and believe me i just send my picture to him and that of my husband and after 48 hours as he have told me, i saw a car drove into the house and behold it was my husband and he have come to me and the kids and that is why i am happy to make every one of you in similar to visit this man via Email and have your lover back to your self Dr. Ekpiku of Ekpikuspelltemple@live.com

  5. joelmercy Reply | Permalink

    Today has being the most happiest day of my life after 1 year of sadness and sorrow without being with the one i love, i tried all my possible best to make sure i make my lover happy but it never seems to work out well it was like am doing everything in vain but all thanks to Dr.uzoya for coming to change all my worries and sadness to Joy. i knew the great man when i read some wonderful reviews about Dr.uzoya how he has helped a lots of people on there relationship problem i was reading a magazine which then i saw great testimonies as well which then i decided not to waste time.he told me not to worry he assures me that within 48 hours everything would be sorted out i believed Dr.uzoya so much because i believe he can't fail me and i sent him all my details. Truly Dr.uzoya never failed me,my husband who left me for good a year come back to me. My husband and i have been living contentedly since this spell caster reunited us together with his love spells. Thank you so so so much Dr.uzoya for your powerful spells. expressions are not sufficient to say thank you. here is his email adddress is; driraborspellcaster@gmail.com OR his miobel phone +2348151098492

  6. shally mills Reply | Permalink

    Good day everyone,I AM FROM SOUTH AFRICA I have been suffering from HIV/Aids for over 4 years now, and suddenly, i have spent all my money all to make sure i get healthy all day, I came in contact with a traditional doctor also known as Dr.Trust on a newspaper. who has help much people to cure their HIV and AIDS disease, firstly i taught it was a joke until i contacted him.and he said that if i am ready for this work, i told him yes, and he collected my details and told me that the spell will only take two days.i was unhealthy ling surprise. And truly he called me to quickly go for a medical checkup, which I did, only to find out that I was not with any HIV disease anymore, my friends and families who left me before run back to me. I now found out that God in Heaven is using this man to bless and heal us all, he is a great and powerful man, again I say to you sir, that God Almighty will uplift you and your great work you did for I and other people. Please i will sincerely advice all HIV patient to contact this great powerful man called Dr Trust for your solutions. Ultimatespellcast@gmail.com or Ultimatespellcast@yahoo.com or call him +2348156885231 He is the best spell caster to work with,i promise you that you will get your problems solved.

  7. robin jane Reply | Permalink

    my name is Robin Jane. I am really pleased with this service. I am one of those people who said, “I’ll never call a psychic or a magician or whatever” to help me with my problems – least of all my love problems, but I reached the point where I knew I needed some guidance, and I’m so glad I found this man called dr. Trust. I never in a million years would have thought I’d be writing a letter like this, but when I nearly lost Andrew in one of our stupid,fights (he broke up with me), I thought I had lost everything.i cry all day and think that he will never come back to me again.i read a testimony by a man called Taylor Borg about him saying that how dr. Trust help him to get back is EX wife within 48n hours.I quickly email him.and also get back my husband with his love spell. And when I was at my most desperate,he didn’t take advantage of me. You performed a very good service for a person in true need. I don’t know how you did it, or how this magic works, but all I know is, IT WORKS!! Andrew my husband and I are happily back together, and I’ll always be grateful to dr. Trust email him for any kinds of help is very capable and reliable for help ultimatespellcast@yahoo.com or ultimatespellcast@gmail.com tel +2348156885231

  8. Emma Reply | Permalink

    I am here to give testimony of how i got back my husband, we got married for over 9 years and we had two kids. thing were going well with us and we where always happy. until one day my husband started to behave in a way i could not understand, i was very confused by the way he treated me and the kids. later that month he did not come back home again and he called me that he want a divorce, i asked him what have i done wrong to deserve this from him, all he was saying is that he want a divorce that he hate me and do not want to see me again in his life, i was mad and also frustrated do not know what to do,i was sick for more than 2 weeks because of the divorce. i love him so much he was everything to me without him my life is incomplete. i told my sister and she told me to contact a spell caster, i never believe in all this spell casting of a thing. i just want to try if something will come out of it. i contacted traditional spell hospital for the return of my husband to me, they told me that my husband have been taken by another woman, that she cast a spell on him that is why he hate me and also want us to divorce. then they told me that they have to cast a spell on him that will make him return to me and the kids, they casted the spell and after 1 week my husband called me and he told me that i should forgive him, he started to apologize on phone and said that he still live me that he did not know what happen to him that he left me. it was the spell that he casted on him that make him come back to me. my family and i are now happy again. Thank you Dr. Aluta for what you have done for me i would have been nothing today if not for your great spell. i want you my friends who are passing through all this kind of love problem of getting back their husband, wife , or ex boyfriend and girlfriend to contact traditionalspellhospital@gmail.com and you will see that your problem will be solved without any delay. He cast spells for different purposes like
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    (9) Herbal care
    (10) is the only answer to that your problem of winning the lottery
    Contact him today on: traditionalspellhospital@gmail.com

  9. oliver wealth Reply | Permalink

    This is amazing i am oliver from uk,i am so happy today because of the great work dr odogun has done in my life for saving my family. I got married to my loving husband 3 years ago and we were living a happy life together and everything was moving on smoothly without any problem, 4 months later I went to the hospital for checkup and the doctor confirmed that I am three weeks pregnant with my heart filled with joy and my face full of smile and laughter I went home excitedly to tell my husband the good news and he was very excited about the news and that day is a day I will always live to remember, to cut the story short after I have put to birth a bouncing baby girl he was very happy, but along the line and all of a sudden he started keeping late night and doing things he don't use to do before, and one mourning I confronted him about his new attitude but he did not listen to my advice, but to my greatest surprise I discover my husband have a mistress in his office place and they were seeing each other after work hour. on one faithful night my husband came back home and started packing all his belongings and told me that he is tired of me that he is filing for a annulment not knowing that his mistress has cast love spell on him that was why he was acting strange so I told my friend about what am experiencing at home she told me not to worry about that and she gave me this contact drodogunlovespelltemple@hotmail.com and ask me to contact him that he will help me to bring back my husband in noon less than 12hours but at first i doubted she told me that this doctor is extremely powerful that the doctor has work for her and she confirm him real so when I got home I contacted him and he told me that my husband is under a great love spell but he promise to help bring back my husband which he actually did within the period of 12hours. I was surprise to see my husband in the nest mourning bring back all the things he took out of our house and kneel in front of me and my kids crying asking for my forgiveness saying it is the work of the devil that he did not know what he was doing and I forgive him and today we are living happily together. i thank you sir for the great work you did for me for saving my family, in-case you have similar case or even worst than my own you can contact him with his EMAIL ADDRESS: drodogunlovespelltemple@hotmail.com

  10. Tanasia Reply | Permalink

    BEWARE OF THESE SCAMS!!! DR UDE GRACE IS THE ONLY SPELL CASTER TO TRUST!( greatspellofsolutiontemple@gmail.com) will be of great help to you.
    My Name is Tanasia Jay, I never believed in spell casting but After 4 years of dating my hobby, he left me because I lost my womb . I felt like my life has come to an end, I almost committed suicide, i was emotionally down for a very long time. Thanks to the spell caster called Dr Grace, whom i met online after my friend Tracy told me about how he helped her. She testified about how Dr Grace brought back her Ex in less than 3 days and reversed the effect of her lost womb, and at the end of her testimony she dropped Dr Grace's email address. I decided to give Dr Grace a try. I contacted her via email and explained my problems to her. In just 3 days, my Hubby came begging. We resolved our issues, and we are even happier than before, am pregnant now to God be the glory. Dr Grace is really a gifted woman and i will not stop publishing her because he is a wonderful woman... Come to think of it I didn't pay much and all I have to do is send him little 250 dollars which he used in providing the materials used for the spell. Even my pastor said that God works mysteriously, that some women are used by God to help others. If you have a problem and you are looking for a real and genuine spell caster to solve all your problems for you. Contact Dr Grace anytime, she is the answer to your problems. Here's her contact (greatspellofsolutiontemple@gmail.com)

  11. barry Reply | Permalink

    Hello ,i am Barry from florida,i am 45 years old,i saw a comment posted by Jenny Louis from New York, on how she was free from skin cancer with cannabis CANNABIS OIL by doctor dories, i was diagnosed of HIV infections for the pass two years , i contacted jenny Louis and she told me that this very doctor cures HIV cancer , and he also cures HIV/AIDS too, then i contacted Dr Deloris, so he told me what to do to get healing and free from HIV , so i make provisions for the HERBAL CREAM which i used for two weeks and now just to see that the exact week which doctor dories told me i we be healed i was felling good and healthy , my skin regained, i went for check up in the hospital and my doctor told that me that all the virus disappeared from my blood vessels and normal, then i made a promise to Dr dories that i will testify of his good herbal work to the world, so I will like you to contact him on (dorisespelltemple@gmail.com ) if you have any health issue is believe Dr Doris will help you

  12. Jessca Edwards Reply | Permalink

    Hello My Name is Jessca Edwards from USA,I just want to share my experience with the entire world on how i got my Ex Husband back and saved my marriage, At least i can say to the world that there is a real spell caster on line called Dr. Ofemo i have benefited from the powers fro the Dr. Ofemo because through his help my Ex Husband has been restored back to me and filled with love within 24hours that i contacted Dr.Ofemo i was not having an idea of what to expect but after proper explanation and i followed the instruction that Dr.Ofemo gave and my relationship was reveal back, i guess whosoever that is in need for relationship help or any having any other problem can contact DR.Ofemo you can contact him true this EMAIL ADDRESS: drofemospelltemple@gmail.com,or drofemospelltemple@yahoo.com or you can contact him through his mobile number:+2348163387496

  13. missjou Reply | Permalink

    After being in relationship with JOHN for five years, he broke up with me, I did everything possible to bring him back to me, but all was in vain, I wanted him back so much because of the love I have for him, I begged him with everything i have, I made promises but he refused. I explained my problem to a friend and she suggested that I should contact a spell caster that could help me cast a spell to bring him back but I am the type that don't believed in spells, I had no choice than to try it, I meant a spell caster called oladimejili and I email him, and he told me there was no problem that everything will be okay before 24hours, that my ex will return to me before 24hours, he cast the spell and surprisingly before 18hours, it was around 4pm. My ex called me, I was so surprised, I answered the call and all he said was that he was so sorry for everything that happened, that he wanted me to return to him, that he loves me so much. I was so happy and went to him, that was how we started living together happily again. Since then, I have made promise that anybody I know that have a relationship problem, I would be of help to such person by referring him or her to the only real and powerful spell caster who helped me with my own problem and who is different from all the fake ones out there. Anybody could need the help of the spell caster, his website is (http://oladimejilispellmiraclespelltemple.webs.com) you can email him (oladimejilispelltemple@gmail.com) if you need his assistance in your relationship or anything. CAN NEVER STOP TALKING ABOUT YOU SIR. CONTACT HIM NOW FOR SOLUTION TO ALL YOUR PROBLEMS

    JOHN JOY.

  14. sandra Reply | Permalink

    My Name is sandra, from United Kingdom. I wish to
    share my testimonies with the general public about what
    this man called Dr Oza has just done for me, this man
    has just brought back my lost Ex husband to me with
    his great spell, I was married to this man called Ariel we
    were together for a long time and we loved our self’s but
    when I was unable to give him a child for 2 years he left
    me and told me he can’t continue anymore then I was
    now looking for ways to get him back until a friend of
    mine told me about this man and gave his contact email,
    then you won’t believe this when I contacted this man
    on my problems he prepared this spell cast and bring my
    lost husband back, and after a month I miss my month
    and go for a test and the result stated am pregnant am
    happy today am a mother of a set of twins, thank you
    once again the great Dr Oza for what you have done for
    me.
    if you are out there passing through any problem like:
    (1) If you want your ex back.
    (2) if you always have relationship problem.
    (3) You want to be promoted in your office.
    (4) You want cure for any disease.
    (5) If you want a child.
    (6) You want to be rich.
    (7) You want people to obey your words.
    (8) If you need financial assistance ETC..
    you can contact his email on
    =DR.OZASPELLHERBALHOME@YAHOO.COM or visithttps://doctorozaspellherbalhome.webs.com for more information contact me at=sandravivian5256@gmail.com or call me at +447031922288

  15. Janet Ruben Reply | Permalink

    My name is Janet Ruben and I live in USA...My life is back again... After 3 years of Broken marriage, my husband left me with kids .I felt like my life was about to end and I almost committed suicide, I was emotionally down for a very long time.Thanks to a spell caster called Dr SUKU,his email address:greatsukusolutiontemple@hotmail.com which I meet online, On one faithful day, as I was browsing through the internet and I came across a lot of testimonies about this particular spell caster.Some people testified that he brought their Ex boyfriend back, some testified that he restores Womb,Cure Cancer and Herpes Virus and HIV Cure other sickness, some testified that he can cast a spell to stop divorce and so on. I also come across one particular testimony and it was about a woman called Joyce,she testified about how he brought back her Ex lover in less than 2 days and at the end of her testimony she dropped Dr SUKU mail address.his website: http://greatsukusolutiontemplehotmailcom.webs.com/ After reading all these,I decided to give it a try and I contacted him via email and explained my problem to him.In just 48 hours, my husband came back to me and we solved our issues, we are even happier than before Dr SUKU is really a gifted man and I will not stop testifying him because he is a wonderful man and so powerful... or call his Mobil number or you can add him true is what App:+234874839242 If you have a problem and you are looking for a real and genuine spell caster to solve all your problems. contact him email:greatsukusolutiontemple@hotmail.com

  16. Sharon Matins Reply | Permalink

    Wow: who will ever believe that a herb can cure HIV Virus, i never believe that this will work i have spend a lot when getting drugs from the hospital to keep me healthy,i went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution out so that my body can be okay. One day I was in the office thinking where I can go to get solution. so a friend walked to my office as me why am I so sad and i open up all to her telling her my problem, she told me that she can help me out, she introduce me to a doctor who uses herbal medication to cure HIV VIRUS and she gave me his email, so i mail him.He told me all the things I need to do and also give me instructions to take, which I followed properly. Before I knew what is happening after two weeks To my greatest surprise after patiently going according to instruction given to me by this man called Dr OSAKA and i went for a medical test and the result was negative. I want to say thanks to Dr OSAKA who God have sent to heal is people and God will bless you for the good work you are doing. you can also contact this great and powerful man if you have this problem his via email;drosakamiracletemple@hotmail.com that why am sharing this post to everyone who still have HIV/aids or HSV VIRUS he do it for me will also do it for you.his lot number:+2348059723809 or his email:drosakamiracletemple@hotmail.com

  17. vivica Reply | Permalink

    What a surprise??? i cant believe this is happening to me...my ex left me for another after 4 years of courtship and i was left alone.After so many temptations and trials,i came across the prophet that brought and he gave me some instructions and i simply abide to it,low and behold it was like a dream when my man called and was begging me to forgive him hahaha so funny and surprising. please hasten up and call him immediately via: prophetajege@gmail.com/+23408071147328

  18. joan Reply | Permalink

    i cant just stop thanking this great man for what he has done in my life because without him i dont know were i would have been now thank you drzizi for bringing back my husband to me if you are out there and you are having any issue with your relationship or you want your lover back or any problem at all you can contact this great man through his email@drzizitemple@gmail.com
    THANKS DRZIZI

  19. Rose James Reply | Permalink

    I never use to believe in spell casting until i met Dr Oga a powerful spell caster who helped me to be a happy person again. My name is Rose James and i reside in USA. After 4 years of Broken marriage, my husband left me with two kids. I felt like my life was about to end and i almost committed suicide, i was emotionally down for a very long time. Thanks to a Great spell caster called Dr Oga which i met online on one fateful day when I was browsing through the internet, i came across a lot of testimonies about this particular Great spell caster how he has helped so many people. he has helped people to bring back their Ex lover, some testified that he restores womb, cure cancer and other sickness, and so on. I also came across a testimony, it was about a woman called Anita, she testified about how his spell made her to be pregnant after so many years of bareness and at the end of her testimony she dropped Dr Oga's email address. After reading all these, i decided to give it a try and i contacted him and explained my problem to him and he assured me that in less than 48 hours, my husband will call me and beg for forgiveness but i thought it will not work. When he had finished casting the spell, the next day my husband called me and he was begging for forgiveness just as Dr Oga said. This is not brain washing and after the spell has been cast, i realized that my husband love me like never before and the spell caster opened him up to know how much i love him and how much love we need to share. We are even happier now than before. Dr oga is really a gifted man and i will not stop publishing him because he is a wonderful man. If you have a problem and you are looking for a real and a genuine spell caster to solve all your problems contact Dr Oga now on Drogaperfectspells@yahoo.com or just visit Drogaperfectspells.blogspot.com and see so many he has helped that are testifying about his good work. He will help you solve all your problems. Once again thank you Dr Oga for your good deeds.

  20. Craig Reply | Permalink

    Came back from prison and found out that my woman was no more.
    Was in a fight at a night club and was sentenced to do 6 months. will just summarize my story.
    The truth be said she stood by me. my woman would always come check on my in prison. when i finnally got out i found out that there was this guy and the guy wanted her to move in with him. my first 2 weeks on the outside my woman would not allow me touch her. i knew something was wrong so i searched for solution online that was how i met dr Bright he told me the guy used something on her and that she was trying to fight it that is why i still have little feelings for me that there was no time we needed to do a reunion spell and a spell removal. to cut the story short he did that and everything returned back to normal.g
    if not for dr bright dont know what i would have done i promised to testify to his good work and that is why i am posting on every forum that dr bright is the best man for your job contact him for you relationship issues on the follwoing:email: brightlovetemple@gmail.com,cell phone: +2348111957679.
    Name: Craig Peterson
    Location: Washington USA

  21. sonia Reply | Permalink

    My name is sonia, I am here to give my testimony about a doctor who helped me in my life. I was infected with HERPES SIMPLEX VIRUS in 2010, i went to many hospitals for cure but there was no solution, so I was thinking how can I get a solution out so that my body can be okay. One day I was in the river side thinking where I can go to get solution. so a lady walked to me telling me why am I so sad and i open up all to her telling her my problem, she told me that she can help me out, she introduce me to a doctor who uses herbal medication to cure HERPES SIMPLEX VIRUS and gave me his email, so i mail him. He told me all the things I need to do and also give me instructions to take, which I followed properly. Before I knew what is happening after two weeks the HERPES SIMPLEX VIRUS that was in my body got vanished . so if you are also heart broken and also need a help, you can also email him at: draigbehispellhome@gmail.com

  22. sandra Reply | Permalink

    first of all let me start by saying that i can not still believe my eyes that my ex that left me for another lady is back to my arms this was made possible through the help of this great man called drzizi and am here letting the whole world know what this great man is capable of doing because he suprized me with his powers if you are out there having problems getting your ex back or you are a cancer patient or having any other problem or you have been childless you can contact this great man through his email @drzizitemple@gmail.com

  23. Ella Philip Reply | Permalink

    HELLO EVERYBODY ella philip FROM CANADA, I AM FULL OF JOY FOR WHAT DR fara DID FOR ME, I NEVER TAUGHT SPELL CASTING WAS REAL BECAUSE I HAVE CONTACTED ALLOT OF SPELL CASTERS AND THEY ALL COLLECTED MY MONEY WITHOUT GIVING ME RESULT, SOMEBODY TOLD ME OF A DR CALLED fara SO I GAVE IT A TRY, BEHOLD! I WAS SURPRISED OF EVERYTHING MY HUSBAND LEFT ME FOR 4YRS BUT DR fara TOLD ME NOT TO WORRY THAT HE WILL RETURN HIM BACK TO ME . TODAY AM THE MOST HAPPIEST WOMAN IN THE WORLD BECAUSE DR fara RESTORE JOY BACK TO ME BY BRINGING HIM BACK, AM GRATEFUL FOR EVERYTHING BECAUSE I NEVER TAUGHT I WILL EVER FEEL THE WARM HANDS AND TOUCH OF MY HUSBAND AGAIN, I PROMISED HIM THAT I WILL ALWAYS SINGS HIS PRAISES EVERYWHERE I GO AND TELL THE WHOLE WORLD ON HOW POWERFUL HE IS…. CONTACT HIM THROUGH THIS EMAIL FOR ALL THOSE OF YOU WHO WANTS TO BE Happily LIKE ME AGAIN.. Email: drfaraspelltemple@gmail.com
    ( DRFARASPELLTEMPLE@GMAIL.COM )
    THESE ARE THE THINGS Dr FARA has remedies to, HERPES . HIV/AIDS . CANCER.LOVE SPELL ON HOW TO GET YOUR HUSBAND BACK E.T.C

  24. Oriel Martins Reply | Permalink

    Greetings to the general public, i want to inform the public how i was cured of HERPES Simplex Virus by a Doctor called Osas. i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Doctor Osas cured them. I Was scared because i never believed in the Internet but i was convince to give him a try because i was having no hope of been cured of HERPES so i decided to contact him on his email that was listed on the comment (doctorosasherbalhome@gmail.com ) i searched his email on net and i saw a lot of people testifying about his goodness. when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HERPES result came out negative. I pray for you Dr Osas God will give you everlasting life, you shall not die before your time for being a sincere and great man. Am so happy, you can also contact him if you have any problem Email: doctorosasherbalhome@gmail.com or contact his number via +2348112252378 or you can as well add him on whatsapp with same number

    DOCTOR OSAS CAN AS WELL CURE THE FOLLOWING DISEASE:-

    1. HIV/AIDS
    2. HERPES
    3. CANCER
    4. ALS

  25. Louise Dickson Reply | Permalink

    My name is Louise Dickson i am from {Birmingham City UK) I want to use this possible means to appreciate one man i hold in sincere gratitude and high esteem for his help and his kindness he has rendered to me.This is the reason why i have taken it upon myself to thank this great spell caster called Dr.Ofemo because through his help my life became more filled with love and i am happy to say that my ex lover who has been separated from me for the past weeks came back to me pleading for acceptance from me, This was a shocking event because before i contacted Dr.Ofemo i was the one begging my ex lover to come back to me but through the assistance of Dr.Ofemo i now have my relationship restored. You can also have a better relationship only if you contact: drofemospelltemple@gmail.com and I’ll always be grateful to dr. Ofemo email him for any kinds of help is very capable and reliable for help drofemospelltemple@yahoo.com or call his telephone number:+2348163387496

  26. Melody Austin Reply | Permalink

    my heart is overwhelmed with Joy.that is why i am advising any one out there to contact Dr Bully, the mighty Psychic that can help you reunite you and your ex lover in just 48 hours...i don't have much to say but i must thank Dr Bully for helping me reunite my marriage with my Ex Husband in 48 hours when i taught nothing could be done any more...he is the best, genuine and Great Psychic that can help you solve any relationship problem, getting pregnant and even help you Cure Any Disease with his Natural Power....his spell work within 48 hours and it has no side effect...you can contact Dr Bully through his email at bullytemple@yahoo.com..you can also add him on Facebook as "Kareem Abhida" or message him through his page on Facebook as "Dr Bully Temple"""...once again his email address is bullytemple@yahoo.com contact him now and get your problem solved for ever...

  27. Jason Reply | Permalink

    Greetings to the general public, i want to inform the public how i was cured of HERPES Simplex Virus by a Dr Ebosa i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr Ebosa cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HERPES result came out negative. I pray for you Dr Ebosa God will give you everlasting life, you shall not die before your time for being a sincere and great men. Am so happy, you can also contact him if you have any problem Email: ebosaherbalremedy@hotmail.com OR or drabosaherbscure@gmail.com
    just call him +2347059498969

    Jason

  28. Jessica Luis Reply | Permalink

    My name is Jessica Luis am from USA, After 1/5 year relationship with my boyfriend, he changed suddenly and stopped contacting me regularly, he would come up with excuses of not seeing me all the time. He stopped answering my calls and my sms and he stopped seeing me regularly. I then started catching him with different girls several times but every time he would say that he love me and that he needed some time to think about our relationship.But cannot stop thinking about him so i decided to go online and i saw so many good talk about this spell caster and i contact him and explain my problems to him.He cast a love spell for me which i use and after 2 day, my boyfriend came back to me and started contacting me regularly and we moved in together after a few months and he was more open to me than before and he started spending more time with me than his friends. We eventually got married and we now have been married happily for 1 years with. Ever since Dr.ocusodo helped me, my partner is very stable, faithful and closer to me than before.You can also contact this spell caster and get your relationship and any kind of problem okay hes email< drocusodospellcaster@gmail.com or drocusodospellcaster@yahoo.com, you can also contact his number +2348105578036

  29. Steph Brian Reply | Permalink

    Been diagnosed of herpes is just like been through hell but EVERYTHING IS POSSIBLE. So amazing and i want to testify of the good work done by GREAT BABA a very strong and powerful spell caster. GREAT BABA has proved science and scientists wrong for believing and thinking that there is no cure for Herpes Virus but to be sincere this GREAT MAN is so real and powerful. I never thought that this cure will work but this GREAT MAN has proved it to me and it has worked and still working perfectly. My Name is Steph Brian and i was diagnosed of the deadly disease called Herpes july 2015 and i thought that, this will be the end and there will be no hope. Speaking to anyone was always a problem because i was so worried and always in the state of unrest in fact i thought it was over until one blessed day when i was searching for help here on the internet, i came across a testimony about GREAT BABA on how he helped a lady who was also infected with Herpes Virus, how he has also helped many others to get their loved ones back, and how he has also help others to cure h i v. At first i thought it was all a lie but i decided to give it a try since there was no option and, when i contacted this MAN he laughed and told me that, that is too small for him to handle but i was still in doubt, and he gave me a form to fill which I did, then he called me and told me that his gods required some items in which he will use in preparing the cure for me. He bought the items and prepared a cure for me, and instructed me on what to do and i OBEYED him completely as he told me. Then he sent a parcel to me through the courier delivery service. it was a holy water and a pure holy oil as described by GREAT BABA. I took the holy water and the holy oil and after taking it, he told me to go for check up but I was still in doubt and was so afraid to go for the test but i took a bold step and believed that it is well, and i believed all what he has done and all that he has told me. So i went to the hospital for the test it was so amazing, awesome and exciting what seems to be impossible became possible, the VIRUS disappeared completely from my system quickly I called him and told him what happened he congratulated me and today i am a happy person again the way things use to be. Don't you think that this testimony is worth sharing? I promise to tell the world about him and i will keep sharing this testimony till the end because many people has died of herpes and i want many people who believed that it is over to know that EVERYTHING IS POSSIBLE AND I WANT THEM TO GET HELP FROM THIS SPELL CASTER by contacting him on his email at Highersolutiontemple@yahoo.com and you can also visit greatbabaofsolution.blogspot.com and see so many people he has helped in other areas of life. Thank you GREAT BABA and May your good gods keep rewarding you for your good deeds.

  30. Kathy Dickson Reply | Permalink

    My name is Kathy and I Want to tell the world about a great man called Dr.Ekpen of EKPEN TEMPLE for bringing joy into my marriage after 3years of divorce from my husband and my 2 kids, i have don everything to bring them back to my life because i love them so much so a friend introduced me to a spell caster last month who did every thing spiritually and bring them back within 48hours, now we are together and happy even more than we where before. You can contact this great spell caster to solve your own relationship problems via email like i did on: (ekpentemple@gmail.com) OR call +2347050270218

  31. kevin anthony Reply | Permalink

    Reasons Why I Can Say Love Spells And other Kinds Of Spell Works
    Reading about spell is not new to anyone who uses the internet frequently.More accurately, most of us depend greatly on the internet for information's and ways to fix things.It can be relationship related, or finance related or any kind of problem you can think of.So in all words we try everything we read to fix it which might just include spell casting.I know this sounds crazy but believe me spell casting is just as real as any other thing you can think of. It just turned out that the internet is flooded with a lot of fake making it is very difficult to separate the "shaft from the wheat" fake from real so here are reason that i think will convince positively about spell casting.
    Even before any of us reading this article was brought into exist those before us and before them and before those before them relied on spell for healing, protection, blessing and all other thing and guess what? they never complained about the failure maybe because there where no fakes then or those who asked the spell casters help completed the process rather than not seeing it through to the end.
    A lot of supernatural thing happens around us.We are either too blind to see it or we just let science explain it for us.We think anything that science can't explain is left to be thought as nothing.
    There are a lot of those all over the internet.Yes, we can say not all are true but yet again, we can't say that they are all not true because we can never be sure unless we go through the complete process that is required from us.
    A lot of you reading this might say am sick or crazy or what ever you may please just describe how discontent you are with this article but am glad even one has his or her right to their personal opinion which this is.It can only be understood by those who see reasons with me.I was in a messed up situation and the only help i could get was from a spell caster and if i have to, will seek their help all over again because even if we refuse to believe,this world is bigger that what our eyes can see and what our senses can feel.
    Even if this sounds religious, it will interest you to know that it is not religious because contrarily to what we all know, Magic or sorcery or spell making has nothing to do with religion its more like the understanding of deep nature that goes beyond what we see and what we feel.If it is right to believe that the earth rotates because some man made telescope tell us so,why is it wrong to believe magic sorcery or spells let us gain access to deep mother nature when nature expose itself everyday.
    This is not meant for everyone to read but if you do,and you want a spell caster find one carefully and if i would,i advice you contact priest jaja with this email that he uses in helping alot of people, it happens i was helped by him and he has been my spiritual father and will also be my spiritual father his email is jaja12demonspell@gmail.com and his phone number is +2347051731432 i wish you best of lucks.

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