The questions and answers below relate to the Research Techniques Made Simple article  "Next Generation Sequencing: Methodology and Application," published online with the August 2013 issue of JID.

Correct answers appear in bold underline


Multiple Choice Questions & Answers:


1.     The basic methodological steps of NGS include:

a)     Template preparation, emulsion PCR, sequencing, data analysis

b)     Template preparation, sequencing and imaging, data analysis

c)     Template amplification, sequencing and imaging, data analysis

d)     Template preparation, sequencing and imaging, alignment to a reference genome

e)     DNA fragmentation, sequencing, data analysis



2.     Advantages of targeted sequencing as opposed to full genome, exome, or transcriptome sequencing include:

a)     Affordable and efficient for quickly interrogating particular genomic regions of interest

b)     Provides a deeper coverage of genomic regions of interest

c)     Can be utilized in deciding a therapeutic plan of action for both germline and somatic cancers

d)     Detects and quantifies low-frequency variants such as rare drug-resistant viral mutations (e.g., HIV, HBV or microbial pathogens)

e)     All of the above


3.     Applications of NGS in medicine include:

a)     Detecting mutations that play a role in diseases such as cancer

b)     Identifying genes responsible for inherited skin diseases

c)     Determining RNA expression levels

d)     Identifying novel virulence factors through sequencing of bacterial and viral species

e)     All of the above

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